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Monoclonal antibodies to human kappa and lambda light chains suitable for staining of formalin-fixed, paraffin-embedded tissue
Sergei I. Syrbu1, Michael B. Cohen2
https://doi.org/10.52645/MJHS.2024.4.01
The hallmark of most B-cell neoplasia is proliferation of B-cells and clonal rearrangement of the immunoglobulin gene, which is lambda or kappa light chain restricted. Since fresh tissue is not always available to determine clonality, immunohistochemical (IHC) staining for kappa and lambda light chains is often performed on formalin-fixed, paraffin-embedded (FFPE) tissue. Commercially available polyclonal antibodies typically produce high background staining and have a significant false negative rate, which makes it difficult to prove clonality. We developed a series of monoclonal antibodies to human kappa and lambda light chain constant regions by immunizing mice with purified kappa and lambda immunoglobulin light chains.
Postoperative urinary retention - prevalence and risk factors: prospective, cohort study
Natalia Belîi1,2*, Cătălina Lozan1
https://doi.org/10.52645/MJHS.2024.3.04
The prevalence of postoperative urinary retention in a single-center surgical population from the Republic of Moldova varies between 5.5% and 7.9%, depending on the criteria for postoperative urinary retention applied. This variation highlights the need for a consensus on diagnostic criteria for postoperative urinary retention is needed. Patients with hypertension and diabetes mellitus were more likely to develop postoperative urinary retention. Patients with pre-existing neurological disorders such as positive history for stroke and diabetic polyneuropathy were more susceptible for postoperative urinary retention.
Deep endometriosis – impact on infertility, endometriosis fertility index, and reproductive prognosis (comparative study)
Nadejda Codreanu*, Elena Ivanova
https://doi.org/10.52645/MJHS.2024.3.03
EFI in patients with DIE does not reflect all aspects of infertility and has reservations, and consideration of both physical symptoms and sexual health is crucial in managing DIE to optimize fertility outcomes. These findings open the way to the feasibility of surgical treatment of DIE to improve sexual quality of life, which will reduce the need for IVF and increase the chances of spontaneous pregnancy in patients, but this conclusion requires further investigation in randomized clinical trials.
Childbirth at advanced reproductive age: the impact of biopsychosocial factors on the mode of delivery
Rodica Scutelnic1,2*, Larisa Spinei1
https://doi.org/10.52645/MJHS.2024.3.02
Advanced reproductive age has been associated with a higher risk of cesarean section compared to vaginal delivery. The influence of age is modulated by various sociodemographic, medical, and obstetric characteristics, including area of residence, education level, history of cesarean section, parity, pregnancy and delivery complications, pre-existing chronic conditions, antenatal care and provision of information on risk factors.
Laparoscopic peritoneal lavage – a new treatment strategy in spontaneous bacterial peritonitis and liver cirrhosis
Gheorghe Anghelici1,2, Tatiana Zugrav1,2*, Sergiu Pisarenco1, Oleg Crudu2, Gheorghe Lupu2, Ana Apascaritei1
https://doi.org/10.52645/MJHS.2024.3.01
The laparoscopic approach in spontaneous bacterial peritonitis in patients with decompensated liver cirrhosis allows for better sanitation of the abdominal cavity, improves peritoneal absorption, and deserves establishment as clinical practice for patients with ascites and peritonitis and cirrhosis.
Statistical and general data on thyroid carcinoma associated with autoimmune thyroiditis in the Republic of Moldova
Early diagnosis of thyroid cancer is difficult because the neoplasm coexists or develops against the background of thyroid nodulo-inflammatory pathologies, which have a slow evolution. The association of thyroid carcinoma with autoimmune thyroiditis remains debatable. In the literature, this combination is noted on average from 1% to 75% of cases. In recent years, there has been an increasing trend in the number of cancers of the thyroid gland against the background of autoimmune thyroiditis. The epidemiological features and morbidity of thyroid cancer associated with lymphocytic (autoimmune) thyroiditis in the Republic of Moldova are not fully elucidated.
Clinical heterogeneity in the early diagnosis of psoriatic arthritis
Eugeniu Russu1*, Liliana Groppa1,2, Lia Chișlari1, Victor Cazac1,2, Larisa Rotaru1,2
https://doi.org/10.52645/MJHS.2022.1.08
PsA are included in the group of HLA-B27-associated joint diseases, united in the group of seronegative spondylitis (SSA). At the same time, this disease differs from ankylosing spondylitis and other spondylitis with a particularly diverse clinical picture and the existence of only the syndromes inherent in it, for example, intermittent synovitis, palindromic rheumatism or mutilating arthritis.